Paul Darby M.S.

8458 Menkar Rd · San Diego, CA 92126 · (858) 337-7981 · pauld0001@gmail.com

 

 

GEN-PROBE

Associate Scientist, Oncology                                                                                                               2005 – Present

• Quantitative Assay feasibility and development of PCA3/PSA triplex system. 
• Real-Time ”Panther” Assay Integration, responsibilities included integrating PCA3/PSA triplex real time assay onto next generation sample handling and detection platform, ensure clinical and amplification performance is consistent with manual assay formats.
• Support the design and feasibility for Hepatitis C Virus real time quantitative assay covering six subtypes. .
• Feasibility design and testing for Gardnerella Vaginosis molecular test. Design and test assay to meet clinical laboratory specificity and dynamic range requirements.
• Biomarker research, design and test new biomarker assay systems:
  • ETV1 quant assay
  • Slc45A3-Erg
  • Slc45A3-Elk4
  • Tmprss2-Erg  (A, B, C, D and E  gene fusions uniplex and multiplexing assays systems
  • Micro-RNA’s (Mir-802), Novel amplification system for small RNA targets.
• Lead designer for the Tmprss2-ERG gene fusion Real-Time Triplex assay system:
  • Designed probes sequences to successfully detect Tmprss2-ERG down to 20 cps/rxn in difficult high GC content regions
  • Demonstrated equivalent clinical performance to manual assay systems
• Designed custom programs in C# to accelerate data analysis.

 

Research Associate III, Assay Feasibility                                                                                                    2001 – 2005

·         Involved in early feasibility and development of Real-Time amplification and detection systems, work included initial feasibility, oligo designs, methods, nomenclature and procedures for Gen-Probe Real-Time technology.  

o    HIV, HCV, West Nile, Influenza A/B, Group A Streptococci, Standardized Internal Control

·         Led evaluation of several OEM instruments to develop real-time amplification chemistries.

·         Basic cloning experience, PCR, qPCR , DNA/RNA extraction and isolation.

 

Research Associate II, Development                                                                                                             1997 – 2001

·         Developed unit dose reagents for use on the Vidas amplification and detection system.

·         Responsibilities included the testing of development and pilot lot reagents for three different assay systems: Chlamydia, Neisseria Gonorrhoeae, Mycobacterium Tuberculosis.

·         Developed HPLC QC methods to determine lot to lot variances in amplification reagent.

·         Developed enzyme kinetic assays to determine quality of enzyme reagents that improved stability and performance of enzyme reagents for the automated platform.

·         Completed Design of Experiment (DOE) studies to improve amplification reagent performance in Whole Urine specimen types for the Neisseria Gonorrhoeae assay.

·         Experience working with BSL3 level organisms.

 

LONG BEACH STATE UNIVERSITY                                                                                                       

Research Associate                                                                                                                                            1993 – 1997

·         Investigated IF-protein’s NF-L, NF-M and NF-H oligomer structure and composition using cross-linkers.

·         Utilized protein purification techniques using Anion and size exclusion chromatography for the isolation of NF-H, M and L IF proteins.

·         Graduate Project: Spectroscopic studies on the effect of Tail domain phosphorylation on the conformation of NF proteins.

·         Teachers Associate for Chemistry 101, Biochemistry Laboratory and Human Anatomy

                                                                                                         

Long Beach State, Long Beach CA,                                                                B.S. & M.S. Biochemistry

·         Compositions and methods for detecting West Nile Virus

·         Method for detecting West Nile Virus nucleic acids in the 5’ non-coding/CAPSID region

·         Compositions and methods for detecting Group A Streptococci

·         Compositions and assays to detect Influenza Virus A and B nucleic acids

·         Compositions and methods to detect Tmprss2/ERG transcript variants in prostate cancer

 

·         Excellence in Innovation Award for Development of Quantitative  Real Time Multiplex Technology

·         Technical Award for Quantitative Real Time Multiplex Tmprss2/PSA Assay

·         C# Certificate, UCSD Extension

·         Visual Basic, C/C++, Java, Perl, ASP.Net, WPF, Visual Studio 2010, Microsoft Expression Blend